As UHPLC has gained a solid foothold in the market, some of the problems that have plagued chromatographers – problems like band broadening, split peaks, carryover, etc. – have begun to occur with greater frequency. While numerous things may be contributing to these problems, one common thing is often overlooked: possible problems with the system’s tubing connections. Problem #1 – Tubing Slippage Because system pressures in UHPLC can exceed 15,000 psi (1,034 bar), the physical demands on the tubing connections can be very significant. Due to the higher pressures of UHPLC coupled with the pressure cycling that can occur in…[Read this article]